Measurement of Free Light Chains in Urine

Measurement of free light chains in urine.

Radioimmunoassay of free light chains of immunoglobulins in urine.

We describe radioimmunoassays for kappa and lambda light chains of immunoglobulins in urine. The assays, which involve antisera to free light chains, were sufficiently sensitive to measure the light chains in unconcentrated urine from healthy subjects. The usefulness of the assays in clinical practice is illustrated by measurements of light chain excretion by patients, including serial studies ...

Immunochemical quantification of free light chains in urine.

kIU/L; normal values 18 kIU/L) autoantibodies, and 2 were negative for all anti-phospholipid antibodies investigated by the ELISA method. Thus, the new SCT assay was positive in 87% of those who had a positive result by our LA test and was negative in 93% of those whose results were negative by our LA assay. All of these patients had histories of thromboembolic disease (4 with venous thrombosis...

Measurement of immunoglobulin free light chains in serum.

but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) ␮g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...

Identification of amyloidogenic light chains requires the combination of serum-free light chain assay with immunofixation of serum and urine.

BACKGROUND The diagnosis of systemic immunoglobulin light-chain (AL) amyloidosis requires demonstration of amyloid deposits in a tissue biopsy and amyloidogenic monoclonal light chains. The optimal strategy to identify the amyloidogenic clone has not been established. We prospectively assessed the diagnostic sensitivity of the serum free light chain (FLC) kappa/lambda ratio, a commercial serum ...